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An application of PLS, MPLS and LOCAL to a
wide range of protein contents, 16 - 93%.
The LOCAL concept by Shenk and Westerhaus is described and applied here to samples with a wide range of protein content. It is compared to the classic PLS method as well as MPLS by Westerhaus. The software package WinISI 4 is used and demonstrated with this example.
Video by Martin Andersson, 2010
A geometric description of repeated weighting
A theory behind rPLS, recursive PLS or repeated weighting PLS is described. Large absolute values of elements in the regression vector are considered important and elements near zero are considered less important. The elements in the regression vector are used as weights in a new PLS regression. The procedure is repeated.
Video by Martin Andersson, 2009
Simulations of open tubular chromatography
Two analytes (blue and red) are pushed forward inside a tube. The walls of the tube are marked yellow. When the analytes hit the walls, they may or may not stay there for a while. If the analytes have different preferences to staying at the wall, they can be separated. This is chromatography.
Video by Martin Andersson, 1996 and 2011
Simulations of field flow fractionation
While being pushed forward horizontally by a laminar flow, the analytes are also pushed down by a vertical force. Differences in random movement due to dependencies between the analyte and the vertical force will influencing the average vertical positions of the analytes. If the analytes respond differently to the vertical force, they will exit at different times, because the laminar flow is slow near the walls and faster far away from the wall.
Video by Martin Andersson, 1999 and 2011
sondette.com, since 2005